Quentin M. Dudley

Reconstitution of monoterpene indole alkaloid biosynthesis in genome engineeredNicotiana benthamiana

The paper reconstitutes strictosidine biosynthesis in Nicotiana benthamiana by co-expressing 14 enzymes, with MLPL from Nepeta enhancing flux through the iridoid pathway.

Biofoundry-assisted expression and characterisation of plant proteins

This paper presents a workflow for automated DNA assembly and cell-free expression of plant proteins to accelerate functional characterisation.

Cell-free prototyping of limonene biosynthesis using cell-free protein synthesis

The paper introduces a cell-free method called iPROBE to rapidly prototype and optimize biosynthetic pathways for producing chemicals like limonene, increasing production from 0.2 to 4.5 mM in 24 hours.

In vitro prototyping and rapid optimization of biosynthetic enzymes for cellular design

The paper introduces iPROBE, a platform for in vitro prototyping and rapid optimization of biosynthetic enzymes, demonstrating improved production of 3-hydroxybutyrate and n-butanol in microbial cell factories.