Cas9-generated auxotrophs of Phaeodactylum tricornutum are characterized by small and large deletions that can be complemented by plasmid-based genes
Samuel S. Slattery, Helen H. Wang, Daniel J. Giguere, Csanad Kocsis, Bradley L. Urquhart, Bogumil Jacek Karas, David R. Edgell
The paper characterizes Cas9-generated auxotrophs in Phaeodactylum tricornutum, showing they can be complemented by plasmid-based genes and exhibit metabolic changes.
bioRxiv · Apr 12, 2020 · PreprintSynthetic Biology