Bogumil Jacek Karas

Clinical Campylobacter jejuni isolates: genomes and genetic tools

The paper sequences two Campylobacter jejuni strains and develops a plasmid system to enhance genetic tools for studying clinical isolates.

Direct Genome Transfer from Acholeplasma laidlawii to Yeast

The paper demonstrates cloning the genome of Acholeplasma laidlawii into yeast by removing a toxic gene and using cell fusion, achieving successful genomic integration in 13 out of 20 colonies.

Conjugation Based CRISPR Antifungals (COBRA)

The paper develops a conjugation-based CRISPR antifungal platform (COBRA) to deliver gene-targeting machinery from E. coli to yeast, demonstrating effective gene elimination and antifungal activity.

Conjugation-mediated DNA delivery to the filamentous fungus Ustilago maydis

This paper demonstrates a method to deliver DNA from Escherichia coli to the fungus Ustilago maydis using conjugation, showing potential for biofungicide development.

Resolving replication incompatibility between chloroplast and conjugative plasmids in E. coli

The paper investigates incompatibility between a conjugative plasmid and a chloroplast genome in E. coli, identifies the cause, and finds an alternative plasmid for efficient DNA transfer.

Engineering Conjugative Plasmids for Inducible Horizontal DNA Transfer

This study develops inducible conjugative plasmids for controlled horizontal gene transfer to combat antimicrobial resistance, showing increased conjugation efficiency upon induction.

Spheroplasted cells: a game changer for DNA delivery to diatoms

This paper develops optimized DNA delivery methods for diatoms, enabling efficient transformation and genome engineering.

PEG Adjustment Enables Genome Transplantation Using Mycoplasma Mycoides Recipient

This study shows that increasing PEG concentration allows successful genome transplantation using Mycoplasma mycoides as recipient cells, expanding the potential for synthetic biology applications.

Deletion of HindIIR and HindIIIR improves DNA transfer via electroporation to Haemophilus influenzae Rd

This paper shows that deleting HindII and HindIII restriction enzymes in H. influenzae improves electroporation transformation efficiency and presents new plasmids for synthetic biology applications.

Identifying functional multi-host shuttle plasmids to advance synthetic biology applications in Mesorhizobium and Bradyrhizobium

The paper develops genetic tools for Mesorhizobium and Bradyrhizobium, including multi-host shuttle plasmids and conjugation protocols, to enhance synthetic biology applications in nitrogen-fixing bacteria.

Design and assembly of the 117-kb Phaeodactylum tricornutum chloroplast genome

The paper describes two methods for cloning the Phaeodactylum tricornutum chloroplast genome with high efficiency using yeast assembly and presents a strategy for integrating designer cassettes.

An expanded genetic toolbox to accelerate the creation of Acholeplasma laidlawii driven by synthetic genomes

This paper develops genetic tools for Acholeplasma laidlawii to enable synthetic genome-based strain engineering.

De novo synthesis of a conjugative system from human gut metagenomic data for targeted delivery of Cas9 antimicrobials

SLICER: Seamless Loss of Integrated Cassettes Using Endonuclease Cleavage and Recombination in Deinococcus radiodurans

The paper introduces SLICER, a method for seamless genome modification in D. radiodurans by recycling selectable markers to delete multiple R-M system genes.

Telomere-to-telomere genome assembly of Phaeodactylum tricornutum

This paper presents a telomere-to-telomere genome assembly of Phaeodactylum tricornutum using Oxford Nanopore long reads, identifying 25 nuclear chromosomes and improving genetic engineering opportunities.

Conjugation-based genome engineering in Deinococcus radiodurans

This paper develops a conjugation-based system for genome engineering in D. radiodurans, enabling stable plasmid introduction and gene deletion protocols.

Genetic requirements for cell division in a genomically minimal cell

Seven genes, including ftsZ, sepF and five of unknown function, are jointly required to restore near-normal morphology and division in the minimal cell JCVI-syn3.0.

Rapid method for generating designer algal mitochondrial genomes

The paper develops a method to generate designer algal mitochondrial genomes using yeast and E. coli hosts for efficient DNA cloning and testing.

Cas9-generated auxotrophs of Phaeodactylum tricornutum are characterized by small and large deletions that can be complemented by plasmid-based genes

The paper characterizes Cas9-generated auxotrophs in Phaeodactylum tricornutum, showing they can be complemented by plasmid-based genes and exhibit metabolic changes.

Designer Sinorhizobium meliloti strains and multi-functional vectors for direct inter-kingdom transfer of high G+C content DNA

The paper develops S. meliloti strains and vectors to transfer high G+C content DNA to multiple hosts.

Diatom Centromeres Suggest a Novel Mechanism for Nuclear Gene Acquisition

The paper identifies diatom centromeres and shows that non-native DNA sequences can function as centromeres, suggesting a mechanism for foreign DNA acquisition.