Daniel J. Giguere

This paper develops genetic tools for Acholeplasma laidlawii to enable synthetic genome-based strain engineering.

The paper improves functional annotations of metagenomic bacteriophage by combining protein structure prediction and homology search in a naphthenic acid-degrading microbial community.

This paper presents a telomere-to-telomere genome assembly of Phaeodactylum tricornutum using Oxford Nanopore long reads, identifying 25 nuclear chromosomes and improving genetic engineering opportunities.

This paper develops a conjugation-based system for genome engineering in D. radiodurans, enabling stable plasmid introduction and gene deletion protocols.

The paper characterizes Cas9-generated auxotrophs in Phaeodactylum tricornutum, showing they can be complemented by plasmid-based genes and exhibit metabolic changes.

The paper presents a strategy to complete and validate metagenome-assembled genomes using a combination of short and ultra-long reads, resulting in multiple complete genomes from a bacterial community.