A CRISPR-Cas assisted shotgun mutagenesis method for evolutionary genome engineering
M. Zhao, M. Gao, L. Xiong, Y. Liu, X. Tao, B. Gao, M. Liu, F.-Q. Wang, D.-Z. Wei
This paper introduces CARM, a CRISPR-Cas-based method for whole-genome mutagenesis, and demonstrates its application in evolving yeast strains for increased beta-carotene and S-adenosyl-L-methionine production.
bioRxiv · Sep 8, 2021Evolutionary Biology