Precision genome editing using synthesis-dependent repair of Cas9-induced DNA breaks
Alexandre Paix, Andrew W. Folkmann, Daniel H. Goldman, Heather M. Kulaga, Michael J. Grzelak, Dominique Rasoloson, Supriya Paidemarry, Rachel Green, Randall R. Reed, Géraldine Seydoux
This paper describes a method for efficient precision genome editing using linear donor DNAs with ~35bp homology arms for synthesis-dependent strand annealing repair of Cas9-induced breaks.
Proceedings of the National Academy of Sciences · Nov 28, 2017Peer-reviewedSynthetic Biology