BiophysicsbioRxivPreprint
Probing drug-target engagement of soluble Gαi1 protein using the SolThermoBRET thermal shift assay
Oscar Brod, Darya Plevako, Themiya Perera…·Dmitry B. Veprintsev
This paper introduces a high-throughput BRET-based thermal shift assay to measure protein stability and ligand interactions with Gi1 protein without purification.