David A. Sykes

The paper investigates how multiple triggers on an allosteric communication network influence preferential coupling of the CB2R to Gi2 over β-arrestin1, revealing insights into GPCR signaling mechanisms.

This paper develops a TR-FRET-based assay to measure ligand binding kinetics for CB1R and CB2R, finding that D77 enables accurate determination of on- and off-rates for various agonists and antagonists.

This study shows that agonist efficacy at the {beta}2AR is determined by differences in receptor affinity for Gs protein, not ligand binding kinetics.

This paper introduces a high-throughput BRET-based thermal shift assay to measure protein stability and ligand interactions with Gi1 protein without purification.

The paper uses docking and machine learning to identify molecular interactions that determine agonist or antagonist activity of β2 adrenoceptor ligands.

This study measures the kinetic binding properties of antipsychotics at 5-HT2A and D2 receptors and correlates them with extrapyramidal side effects (EPS) at therapeutic doses.