Rapid genome recoding by iterative recombineering of synthetic DNA
Yu Heng Lau, Finn Stirling, James Kuo, Michiel A. P. Karrenbelt, Yujia A. Chan, Adam J. Riesselman, Connor A. Horton, Elena Schäfer, David Lips, Matthew T. Weinstock, Daniel G. Gibson, Jeffrey C. Way, Pamela A. Silver
This paper introduces a method for rapid genome recoding in Salmonella typhimurium by iteratively recombineering synthetic DNA, achieving the highest number of codon substitutions in a single organism.
Nucleic Acids Research · May 2, 2017Peer-reviewedSynthetic Biology