Continual improvement of multiplex mutagenesis in Arabidopsis
Ward Develtere, Ward Decaestecker, Debbie Rombaut, Chantal Anders, Elke Clicque, Marnik Vuylsteke, Thomas B. Jacobs
The paper tests multiple promoters and Cas9 NLS configurations in Arabidopsis to improve multiplex mutagenesis efficiency, finding the RPS5A promoter and double-BP NLS to be most effective.
bioRxiv · Dec 19, 2023 · PreprintSynthetic Biology