Thomas B. Jacobs

This study identifies Arabidopsis BAG proteins as redundant proteostasis factors that maintain CESA6 stability during salt stress, ensuring cellulose synthesis and plant stress tolerance.

The paper introduces B3, a modular Golden Gate system for assembling Agrobacterium binary vector backbones, enabling efficient and flexible construction of over 370,000 possible constructs.

Fluorescent tagging of 160 proteins in the seaweed Ulva maps its carbon-concentrating pathway, and identifies SUPA1, a Rubisco linker that binds only four of eight sites.

This study identifies SCAMP proteins as regulators of aquaporin abundance in Arabidopsis root cells, showing reduced PIP levels in mutants lead to improved drought response.

This paper expands the molecular toolkit for Ulva compressa by developing efficient gene editing and selection systems for functional genomics studies.

The paper tests multiple promoters and Cas9 NLS configurations in Arabidopsis to improve multiplex mutagenesis efficiency, finding the RPS5A promoter and double-BP NLS to be most effective.

This paper introduces a method called oligo stitching that enhances Golden Gate cloning by using single-stranded DNA oligos to convert parts between standards and assemble incompatible parts without PCR.

The paper develops an optimized LbCas12a-ABE base editor in wheat and maize using the ITER platform, achieving 40% activity on a GFP reporter through iterative design.

BREEDIT improves complex traits in maize by combining CRISPR/Cas9 gene editing with crossing schemes to generate mutants with enhanced growth characteristics.

The paper shows that knocking down Tnt1 expression in tobacco leads to transcriptomic and phenotypic changes, suggesting Tnt1 regulates gene expression and plant development.