Author

Divya Kolakada

University of Colorado Anschutz

Listed by name: these papers carry no ORCID for this author, so different people with the same name may be mixed together.

Biology Disciplines

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Molecular Biology· 2 Bioengineering· 1

Signature Assays, Organisms & Methods

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Approach:experimental assay· 2 Research Focus:benchmarking· 1 Assay:bulk RNA-seq· 1 Assay:CRISPR screen· 1 Research Focus:differential expression· 1 Research Focus:gene regulatory network inference· 1 Organism:human· 1 Organism:mouse· 1

Frequent Co-Authors

Amy E. CampbelliD· 2 shared Mlana Lore· 2 shared Sujatha JagannathaniD· 2 shared Alexey V. ShuvaloviD· 1 shared Derek K. SmithiD· 1 shared Elena Z. AlkalaevaiD· 1 shared Jay R. HesselberthiD· 1 shared Joanna L. Donatelli· 1 shared

Activity by Year

2024-2025 · peak 2/yr

Co-Author Collaboration Network

10 top collaborators · 25 mutual co-author links

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2 shared papersMolecular Biology

Amy E. CampbellORCID

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3 papers

Recent·Most cited

An Efficient Workflow for CHO Cell Genome Engineering with OpenCRISPR-1

Divya Kolakada, Ramya Ankala, Joanna L. Donatelli, Derek K. Smith

This paper validates OpenCRISPR-1 in CHO cells, achieving ≥70% INDEL efficiency across multiple genes using a 39-day workflow.

bioRxiv · Dec 20, 2025 · PreprintBioengineering

Systematic analysis of nonsense variants uncovers peptide release rate as a novel modifier of nonsense-mediated mRNA decay

Divya Kolakada, Rui Fu, Nikita Biziaev, Alexey V. Shuvalov, Mlana Lore, Amy E. Campbell, Michael A. Cortázar, Marcin Piotr Sajek, Jay R. Hesselberth, Neelanjan Mukherjee, Elena Z. Alkalaeva, Zeynep Coban‐Akdemir, Sujatha Jagannathan

The paper identifies the amino acid preceding a premature termination codon as a key determinant of nonsense-mediated mRNA decay efficiency.

Cell Genomics · May 19, 2025Peer-reviewedMolecular Biology

A system of reporters for comparative investigation of EJC-independent and EJC-enhanced nonsense-mediated mRNA decay

Divya Kolakada, Amy E. Campbell, Laura D. Baquero Galvis, Zhongyou Li, Mlana Lore, Sujatha Jagannathan

The paper introduces a reporter system to compare EJC-independent and EJC-enhanced NMD, showing differences in RNA and protein levels and varying readthrough responses to inhibitors.

Nucleic Acids Research · Feb 7, 2024Peer-reviewedMolecular Biology