Direct detection of circulating microRNA-122 using dynamic chemical labelling with single molecule detection overcomes stability and isomiR challenges for biomarker qualification
B. Lopez-Longarela, E. E. Morrison, J. D. Tranter, L. Chahman-Vos, J.-F. Leonard, J.-C. Gautier, S. Laurent, A. Lartigau, E. Boitier, L. Sautier, P. Carmona-Saez, J. Martorell-Marugan, R. J. Mellanby, S. Pernagallo, H. Ilyine, , J. J. Diaz-Mochon
This paper develops a DCL assay to directly detect miR-122 in serum/plasma, overcoming stability and isomiR challenges for DILI biomarker qualification.
bioRxiv · Sep 20, 2019 · PreprintPharmacology & Toxicology