A real-time, transient kinetic study of Drosophila melanogaster Dicer-2 elucidates mechanism of termini-dependent cleavage of dsRNA.
R. K. SINGH, M. Jonely, E. Leslie, N. A. Rejali, R. Noriega, B. L. Bass
This study investigates the kinetic mechanism of Drosophila Dicer-2 processing dsRNA with different termini using stopped-flow and fluorescence techniques, revealing ATP-dependent conformational changes and cleavage dynamics.
bioRxiv · Sep 29, 2020Biophysics