R. K. Singh

Sulfated mannan helps diatoms domesticate their microbiome

This paper shows diatoms exude sulfated mannan to selectively nourish bacteria, shaping their microbiome to enhance productivity and carbon cycling.

Decoding the role of DNA sequence on protein-DNA co-condensation

This paper develops a polymer-based model to study how DNA sequence affects protein-DNA co-condensation, showing that sequence heterogeneity leads to multiple condensates.

LRMP inhibits the cAMP potentiation of HCN4 channels by disrupting intramolecular signal transduction

This study identifies how LRMP inhibits cAMP-induced potentiation of HCN4 channels by disrupting intramolecular signaling, revealing isoform-specific interaction domains.

Fluorogenesis: Inducing Fluorescence in a Non-Fluorescent Protein Through Photoinduced Chromophore Transfer of a Genetically Encoded Chromophore

This paper induces red fluorescence in a non-fluorescent kinase using light-induced chromophore transfer from a photocleavable protein, demonstrating its use as a cyclable high-temperature sensor.

Targeting MAPAKAP2(MK2) to combat inflammation by avoiding the differential regulation of anti-inflammatory genes by p38 MAPK inhibitors

The paper evaluates MK2 inhibitors as a safer alternative to p38 MAPK inhibitors for anti-inflammatory therapy by comparing their toxicity and efficacy in cell-based assays.

Septins Provide Microenvironment Sensing and Cortical Actomyosin Partitioning in Motile Amoeboid T Lymphocytes

This paper identifies septins as key regulators of amoeboid motility in T cells by enabling microenvironment sensing and actomyosin partitioning through steric interactions with collagen fibers.

Systemic infection of SARS-CoV-2 in free ranging Leopard (Panthera pardus fusca) in India

The paper reports SARS-CoV-2 infection in a free-ranging Indian leopard, confirming Delta variant infection and highlighting the need for wildlife screening.

A Binary Matrix Method to Enumerate, Hierarchically Order and Structurally Classify Peptide Aggregation

This paper introduces a binary matrix method to enumerate, hierarchically order, and structurally classify peptide aggregates in MD simulations, outperforming conventional metrics.

Nanobodies as allosteric modulators of Parkinson's disease-associated LRRK2

This study identifies and characterizes Nanobodies that modulate LRRK2 activity through non-ATP-competitive mechanisms, offering new tools for Parkinson's disease research and therapy.

Pathotyping of Newcastle disease virus: A novel single BsaHI digestion method of detection and differentiation of avirulent strains (lentogenic and mesogenic vaccine strains) from virulent virus

The paper introduces a single BsaHI digestion method to detect and differentiate avirulent NDV strains from virulent ones using RT-PCR and sequence analysis.

Mechanical counterbalance of kinesin and dynein motors in microtubular network regulates cell mechanics, 3D architecture, and mechanosensing

The paper shows dynein and kinesin motors balance microtubule network mechanics, affecting cell shape, adhesion, and mechanosensing through opposing actions on microtubule organization.

Characterization of a novel mesophilic CTP-dependent riboflavin kinase and rational engineering to create its thermostable homologs

This study characterizes a mesophilic CTP-dependent riboflavin kinase from Methanococcus maripaludis and identifies residues responsible for thermostability through comparative analysis and mutagenesis.

Vibrio cholerae ParE2 toxin modulates its operon transcription by stabilization of an antitoxin DNA ruler

This study investigates how ParE2 toxin modulates its operon transcription by stabilizing the antitoxin DNA ruler through protein-DNA interactions and structural analysis.

Entropic pressure controls oligomerization of Vibrio cholerae ParD2 antitoxin

This study shows that the intrinsically disordered C-terminal domain of VcParD2 restricts its oligomerization to stable 10-mer or 12-mer structures due to entropic pressure.

Binding of dsRNA by D. melanogaster Dicer-2 is substrate-dependent and regulated by Loquacious-PD

The paper investigates how Dicer-2 binds dsRNA with different termini and how this binding is regulated by Loquacious-PD and ATP.

A real-time, transient kinetic study of Drosophila melanogaster Dicer-2 elucidates mechanism of termini-dependent cleavage of dsRNA.

This study investigates the kinetic mechanism of Drosophila Dicer-2 processing dsRNA with different termini using stopped-flow and fluorescence techniques, revealing ATP-dependent conformational changes and cleavage dynamics.

Targeting Vitamin-D receptor (VDR) by a small molecule antagonist MeTC7 inhibits PD-L1 but controls THMYCN neuroblastoma growth PD-L1 independently

This study shows that MeTC7, a VDR antagonist, reduces PD-L1 expression in cancer cells and inhibits neuroblastoma growth independently of PD-L1.

Role of Sugar Stereochemistry on Structural and Free Energy Landscape of Double-Stranded Nucleic Acids

This study investigates how sugar stereochemistry affects the structural and free energy landscapes of double-stranded nucleic acids using molecular dynamics simulations.

KSHV-encoded vCyclin can modulate HIF1α levels to promote DNA replication in hypoxia

This study shows that KSHV-encoded vCyclin modulates HIF1α levels in hypoxia to promote DNA replication and cell proliferation.

Rub1/NEDD8, a ubiquitin-like modifier, is also a ubiquitin modifier

This paper shows that a single amino acid swap at position 72 can switch the substrate specificity of Rub1/NEDD8 and ubiquitin, revealing their distinct roles in cellular modification pathways.

SARS-CoV-2 Spike Glycoprotein and ACE2 interaction reveals modulation of viral entry in wild and domestic animals

This study evaluates the interaction between SARS-CoV-2 spike protein and ACE2 receptors across species to predict viral entry probabilities in different animals.

Septins disruption controls tumor growth and enhances efficacy of Herceptin

The paper develops UR214-9, a compound that disrupts septin filaments in cancer cells, inhibiting growth of HER2+ tumors and enhancing Herceptin efficacy.

Semecarpus anacardium Linn. leaf extract exhibits activities against breast cancer and prolongs the survival of tumor-bearing mice

This study shows that Semecarpus anacardium leaf extract inhibits breast cancer cell growth and extends survival in tumor-bearing mice.

Molecular characterization and differential expression reveal functional divergence of stress-responsive enzymes in C4 panicoid models, Setaria italica and Setaria viridis

The paper identifies and characterizes stress-responsive genes in C4 plants Setaria italica and S. viridis, highlighting key candidates for abiotic stress tolerance.

SRSF2 regulation of MDM2 reveals splicing as a therapeutic vulnerability of the p53 pathway

This study shows that SRSF2 regulates MDM2 splicing, affecting p53 activity, and suggests splicing modulation as a potential therapeutic strategy for cancer.

DMENet: Diabetic Macular Edema Diagnosis using Hierarchical Ensemble of CNNs

DMENet uses a hierarchical ensemble of CNNs to diagnose and grade diabetic macular edema from retinal images, achieving high accuracy and sensitivity.

Detection and characterization of a novel copper-dependent intermediate in a lytic polysaccharide monooxygenase

The paper identifies a long-lived copper-dependent intermediate in an LPMO enzyme and proposes a mechanism involving its formation from a reactive Cu(III) intermediate during cellulose oxidation.

TLR4-dependent signaling drives extracellular catabolism of low-density lipoprotein aggregates

This study shows TLR4 signaling promotes extracellular catabolism of LDL aggregates and lipid accumulation in macrophages through lysosomal synapse formation.

Overcoming ibrutinib resistance by targeting phosphatidylinositol-3-kinase signaling in diffuse large B-cell lymphoma

This study identifies phosphatidylinositol-3-kinase-beta upregulation as a mechanism of ibrutinib resistance in diffuse large B-cell lymphoma and shows that targeting this pathway with KA2237 can overcome resistance.

Transient kinetic analysis of SWR1C-catalyzed H2A.Z deposition unravels the impact of nucleosome dynamics and the asymmetry of stepwise histone exchange

This study uses transient kinetic analysis to reveal how SWR1C catalyzes H2A.Z deposition, showing asymmetry in ATP-dependent dimer exchange and nucleosome dynamics.