E. T. PAPOUTSAKIS

Similar but distinct: the impact of biomechanical forces and culture age on the production, cargo loading, and biological efficacy of human megakaryocytic extracellular vesicles for applications in cell and gene therapies

This study investigates how biomechanical forces and culture age affect the production and biological activity of megakaryocytic extracellular vesicles (MkEVs), revealing force-dependent differences in miRNA cargo and efficacy.

Kinetic and functional analysis of abundant microRNAs in extracellular vesicles from normal and stressed cultures of Chinese Hamster Ovary (CHO) cells

This study quantifies microRNA and protein levels in CHO cell extracellular vesicles under various stress conditions and assesses their impact on cell viability.

Native and engineered human megakaryocytic extracellular vesicles for targeted non-viral cargo delivery to blood stem cells

This paper demonstrates that native and engineered human megakaryocytic extracellular vesicles can deliver non-viral cargo specifically to hematopoietic stem and progenitor cells, enhancing platelet production in mice.

Polyethylene valorization by combined chemical catalysis with bioconversion by plastic-enriched microbial consortia

This paper combines chemical catalysis and microbial consortia to valorize polyethylene deconstruction products into valuable wax esters.

The microRNomes of Chinese Hamster Ovary (CHO) cells and their extracellular vesicles, and how they respond to osmotic and ammonia stress

This study characterizes miRNA profiles in CHO cells and their extracellular vesicles under normal and stress conditions, revealing shifts in miRNA enrichment associated with osmotic and ammonia stress.

Novel mechanism of plasmid-DNA transfer mediated by heterologous cell fusion in syntrophic coculture of Clostridium organisms

The paper demonstrates a novel mechanism of horizontal gene transfer via heterologous cell fusion in syntrophic Clostridium cocultures, enabling plasmid DNA exchange and antibiotic resistance acquisition.

Extracellular Vesicles Facilitate Large-Scale, Homogenizing Dynamic Exchange of Proteins and RNA Among Cultured Chinese Hamster Ovary (CHO) and Human Cells

The paper shows that extracellular vesicles enable large-scale protein and RNA exchange between different cell types, including CHO cells, human cell lines, and primary human cells.

Expression of soluble methane monooxygenase in Escherichia coli enables methane conversion

This paper demonstrates the conversion of methane to acetone using engineered Escherichia coli expressing soluble methane monooxygenase.

Combinatorial effect of miR-486-5p & miR-22-3p mimics thrombopoietin's impact on hematopoietic stem & progenitor cells

The paper identifies miR-486-5p and miR-22-3p as drivers of megakaryocytic differentiation in HSPCs without thrombopoietin, and shows MkMPs induce this through JNK and PI3K/Akt/mTOR signaling.