Cas12a is a dynamic and precise RNA-guided nuclease without off-target activity on {lambda}-DNA
B. Paul, L. Chaubet, E. Verver, G. Montoya
This study investigates Cas12a's DNA binding and cleavage mechanisms using optical tweezers and fluorescence, showing it cleaves only the target site without off-target activity on λ-DNA.
bioRxiv · Jun 9, 2021 · PreprintBiophysics