G. Montoya

Detuning of the Ribosome Conformational Landscape Promotes Antibiotic Resistance and Collateral Sensitivity

The paper identifies a ribosome mutation that causes antibiotic resistance by altering conformational dynamics and reveals new tobramycin binding sites, leading to collateral sensitivity to chloramphenicol.

Structure of the TnsB transposase-DNA complex of type V-K CRISPR-associated transposon

The paper presents the cryo-EM structure of the TnsB transposase-DNA complex from a type V-K CRISPR-associated transposon, revealing its architecture and catalytic mechanism.

Structure of the RAF1-HSP90-CDC37 complex reveals the basis of RAF1 regulation

The paper determines the structure of the RAF1-HSP90-CDC37 complex using cryo-EM, revealing how CDC37 regulates RAF1 and potential therapeutic targets.

Cellular toxicity of iHAP1 and DT-061 does not occur through PP2A-B56 targeting

This study challenges previous claims that iHAP1 and DT-061 target PP2A-B56 complexes, showing instead that they exert toxicity through microtubule disruption and ER/Golgi disruption, respectively.

Structure of the mini-RNA-guided endonuclease CRISPR-Cas{Phi}3

This paper determines the cryoEM structure of CRISPR-Cas{Phi}3, revealing its DNA targeting and cleavage mechanism, and shows how it enables unspecific ssDNA degradation.

Cas12a is a dynamic and precise RNA-guided nuclease without off-target activity on {lambda}-DNA

This study investigates Cas12a's DNA binding and cleavage mechanisms using optical tweezers and fluorescence, showing it cleaves only the target site without off-target activity on λ-DNA.

A highly conserved binding pocket on PP2A-B56 is required for shugoshin binding and cohesion protection

This study identifies a conserved binding pocket on PP2A-B56 essential for Sgo1 interaction and cohesion protection, showing that complex formation is not strictly required for cohesion protection.

DeepFRET: Rapid and automated single molecule FRET data classification using deep learning

DeepFRET automates smFRET data classification using deep learning, achieving >95% accuracy and speed compared to human operators.

Structures of the Cmr-βComplex Reveal the Regulation of the Immunity Mechanism of Type III-B CRISPR-Cas

This study reveals how target RNA binding regulates Cmr-β complex activities through conformational changes, enabling immune responses against genetic invaders.

Structure Of The Cpf1 Endonuclease R-Loop Complex After Target DNA Cleavage

This paper determines the crystal structure of FnCpf1 after DNA cleavage, revealing how it unwinds DNA and processes the PAM sequence.