Development of SYK NanoBRET Cellular Target Engagement Assays for Gain-of-Function Variants
Jacob L. Capener, James D. Vasta, Vittorio L. Katis, Ani Michaud, Michael Beck, S. C. D. Daglish, Sarit Cohen‐Kedar, Efrat Shaham Barda, Stefanie Howell, Iris Dotan, Matthew B. Robers, Alison D. Axtman, Frances M. Bashore
The paper develops a NanoBRET assay to study SYK gain-of-function variants in live cells and demonstrates inhibitor efficacy against these variants.
Frontiers in Chemical Biology · Jul 29, 2024Peer-reviewedCell Biology