Proxi-RIMS-seq2 applied to native microbiomes uncovers hundreds of known and novel m5C methyltransferase specificities.
Weiwei Yang, Yvette A. Luyten, Emily Reister, Hayley Mangelson, Zach Sisson, Benjamin Auch, Ivan Liachko, Richard J. Roberts, Laurence M. Ettwiller
The paper applies Proxi-RIMS-seq2 to native microbiomes to identify m5C methylation motifs and their cognate methyltransferases, revealing over 1900 motifs including a novel 8-base recognition site.
Nucleic Acids Research · Mar 20, 2025Peer-reviewedGenomics