Laurence M. Ettwiller

The paper identifies 5mC-selective deaminases and demonstrates their use in ultra-sensitive methylome sequencing at single-base resolution.

The paper introduces a 'bait-and-switch' strategy to link enzyme function with community-level phenotypes in microbiomes, demonstrating its feasibility in identifying chitin-degrading enzymes.

This study examines how adding chitin-rich crab and lobster shell compost to salt marsh sediments alters microbial community structure and function, revealing shifts in metabolism and potential pathogen emergence.

The paper uses Cappable-Seq to analyze transcriptional start sites in Wolbachia under stress conditions, revealing differential regulation and insights into gene operon organization.

The paper identifies key players in the 2,6-diaminopurine biosynthesis pathway using metagenomic data from aquatic environments.

This paper introduces a novel enzymatic strategy using 7-deaza-dGTP and Fpg to remove carryover contamination and improve sequencing performance in NGS.

The paper applies Proxi-RIMS-seq2 to native microbiomes to identify m5C methylation motifs and their cognate methyltransferases, revealing over 1900 motifs including a novel 8-base recognition site.

This study introduces RIMS-seq2, a method to simultaneously sequence the genome and methylome with minimal changes to DNA sequencing protocols, showing accurate variant calling and methylation mapping.

The paper identifies new prokaryotic wH domain-containing endonucleases that sense adenine methylation in specific DNA contexts.

This study identifies two independent origins of filarial Wolbachia in supergroup F and shows convergent loss of bacterioferritin in filarial Wolbachia.

The paper reports the first genomes of Mansonella perstans and Mansonella ozzardi, enabling comparative genomic analysis and insights into their biology, evolution, and drug resistance.

The paper identifies two distinct error profiles in FFPE DNA caused by cytosine and methylcytosine deamination and shows how specific repair strategies can mitigate these errors.

Methyl-SNP-seq captures both methylation and sequence information from single DNA molecules, enabling analysis of allele-specific methylation and methyltransferase specificity.

The paper investigates the BREX phage exclusion system in Salmonella, showing that mutations in flanking genes affect methylation and restriction despite intact modification genes.

This study introduces NRCeq, a method to identify capped full-length SARS-CoV-2 transcripts, revealing new insights into sgRNA structure and expression.

ReCappable-seq identifies TSS for all RNA polymerases at single-nucleotide resolution, revealing novel TSS in nuclear and mitochondrial genomes and expanding understanding of non-Pol-II transcription.

This study introduces MetaGPA, a framework linking metagenomic genetic data to functional phenotypes, and identifies a novel cytosine modification mechanism in DNA and RNA.

The paper identifies high-confidence full-length human RNA isoform scaffolds using nanopore sequencing by improving 5' cap detection and 3' polyadenylation site identification.

RIMS-seq identifies methylase specificities and sequences bacterial genomes using a simple protocol, revealing new methylase targets in bacteria and synthetic communities.

This paper introduces LR-EM-seq, an enzymatic deamination method for long-range methylation profiling of 5-mC and 5-hmC, achieving better-defined DMRs over 5 kb.

The paper introduces a computational framework, SeqATU, to predict alternative transcription units in bacterial genomes using RNA-Seq data, achieving high precision and recall in E. coli.

SMRT-Cappable-seq reveals extended operons and read-through termination sites in E. coli, showing most bacterial genes are part of multiple operon variants.