Target-matched increased fidelity SpCas9 variants can edit any target without genome-wide off-target effects
Péter István Kulcsár, András Tálas, Zoltán Ligeti, Eszter Tóth, Zsófia Rakvács, Vanessza Laura Végi, Sarah Laura Krausz, Krisztina Huszár, Ervin Welker
The paper develops a set of high-fidelity SpCas9 variants that enable efficient and specific genome editing across all target sites without off-target effects.
bioRxiv · Aug 16, 2022 · PreprintMolecular Biology