Alanine-scanning of the yeast killer toxin K2 reveals key residues for activity, gain-of-function variants, and supports prediction of precursor processing and 3D structure.
Rianne C. Prins, Tycho Marinus, Eyal Dafni, Iftach Yacoby, Sonja Billerbeck
This study uses alanine scanning and computational methods to map sequence-function relationships in the yeast killer toxin K2, revealing key residues for activity and enabling engineering of enhanced toxicity and specificity.
Journal of Structural Biology X · Dec 19, 2025Peer-reviewedMicrobiology