RNAP-seq: in vitro genome-scale transcription reveals preferential RNA polymerase pausing on Clostridioides difficile antisense DNA
Ping Shen, Michael B. Wolfe, Jason Saba, Rachel Anne Mooney, Yu Bao, Ezaz Ahmad, Robert Landick, Xinyun Cao
The paper introduces RNAP-seq, an in vitro method to study transcriptional pausing, and finds that CdfRNAP pauses more frequently at T-rich antisense regions compared to EcoRNAP.
bioRxiv · May 8, 2026 · PreprintGenomics