Robert C. Landick

This study shows that RNA polymerase inhibitors prevent RH-FL closure, destabilizing the trigger loop and inhibiting the nucleotide-addition cycle.

This study investigates how ppGpp regulates RNA polymerase pausing and nucleotide addition through allosteric effects and GTP competition in E. coli.

The paper introduces RNAP-seq, an in vitro method to study transcriptional pausing, and finds that CdfRNAP pauses more frequently at T-rich antisense regions compared to EcoRNAP.

This study shows that adjusting pH and using SAA pretreatment improves biofuel production from drought-switchgrass hydrolysates.

The paper shows RapA disrupts post-termination complexes in E. coli to prevent R-loop formation and maintain genome stability.

The paper investigates how Bacteroides use the Y-Z regulatory system to control capsule polysaccharide (CPS) transcription and create subpopulations for fitness.

The paper reports cryo-EM structures of E. coli intrinsic termination complexes to define the structural mechanism of bacterial transcription termination.

This study redesigns bacterial RNA polymerase promoter specificity using computational design and screening to create orthogonal sigma factors that recognize new promoter targets.

The paper uses high-throughput single-cell sequencing to show promoter inversion rates drive population heterogeneity in Bacteroides spp.

This study identifies MtbNusG as a pro-pausing transcription factor that stabilizes paused RNAP through specific structural interactions, with implications for NusG function across domains of life.

The paper uses TEN-map to show H-NS DBDs contact DNA at the same irregularly spaced sites in both bridged and linear filaments, supporting a hemi-sequestration model.

The paper uses chemical-genetic analysis of RNA polymerase mutants to uncover structure-function relationships and physiological trade-offs in Escherichia coli.

CRISpy-pop designs and predicts CRISPR/Cas9 guide RNA activity across diverse yeast and bacterial strains, including human genome cross-referencing for biosafety.

The paper investigates the role of RNA polymerase clamp movements in intrinsic termination, finding that clamp opening affects elongation rate more than termination rate and that RNA can release from closed clamps.