Jason Saba

This study investigates how ppGpp regulates RNA polymerase pausing and nucleotide addition through allosteric effects and GTP competition in E. coli.

The paper introduces RNAP-seq, an in vitro method to study transcriptional pausing, and finds that CdfRNAP pauses more frequently at T-rich antisense regions compared to EcoRNAP.

The paper investigates how Bacteroides use the Y-Z regulatory system to control capsule polysaccharide (CPS) transcription and create subpopulations for fitness.

The paper uses high-throughput single-cell sequencing to show promoter inversion rates drive population heterogeneity in Bacteroides spp.