Engineering the thermotolerant industrial yeast Kluyveromyces marxianus for anaerobic growth
W. J. C. Dekker, R. Ortiz-Merino, A. Kaljouw, F. W. Wiering, C. Mooiman, P. de la Torre, J. T. Pronk
This study identifies the absence of a functional sterol-uptake mechanism in K. marxianus as the cause of its oxygen requirement and demonstrates that expressing a squalene-tetrahymanol cyclase enables anaerobic growth.
bioRxiv · Jan 7, 2021Biochemistry