BiochemistrybioRxiv
Engineering the thermotolerant industrial yeast Kluyveromyces marxianus for anaerobic growth
W. J. C. Dekker, R. Ortiz-Merino, A. Kaljouw…·J. T. Pronk
This study identifies the absence of a functional sterol-uptake mechanism in K. marxianus as the cause of its oxygen requirement and demonstrates that expressing a squalene-tetrahymanol cyclase enables anaerobic growth.
DOI 10.1016/j.ymben.2021.07.006