CRISPR/Cas with ribonucleoprotein complexes and transiently selected telomere vectors allows highly efficient marker-free and multiple genome editing in Botrytis cinerea
Thomas Leisen, Fabian Bietz, J.A. Werner, Alex Wegner, Ulrich Schaffrath, David Scheuring, Felix Willmund, Andreas Mosbach, Gabriel Scalliet, Matthias Hahn
This paper introduces a CRISPR/Cas system using ribonucleoprotein complexes and telomere vectors for efficient marker-free genome editing in Botrytis cinerea, achieving high editing rates and enabling multiple edits.
PLoS Pathogens · Aug 17, 2020Peer-reviewedGenetics