The Activome: multiplexed probing of activity of proteolytic enzymes using mass cytometry-compatible activity-based probes (TOF-probes)
M. Poreba, K. Groborz, W. Rut, M. Pore, S. J. Snipas, M. Vizovisek, B. Turk, M. Drag, G. S. Salvesen
The paper develops lanthanide-labeled activity-based probes for mass cytometry to multiplex protease activity detection, demonstrating their use in identifying protease activomes in cell lines and blood cells.
bioRxiv · Sep 23, 2019Biochemistry