Spatial mapping of DNA synthesis reveals dynamics and geometry of human replication nanostructures
Michael Hawgood, Bruno Urién, Ana Agostinho, Praghadhesh Thiagarajan, Giovanni Giglio, Yiqiu Yang, Xue Zhang, Gemma Quijada, Matilde Rodrigues da Fonseca, Jiří Bártek, Hans Blom, Bennie B. L. G. Lemmens
This study introduces 3D-SPARK, a method to visualize and quantify DNA replication dynamics at the nanoscale in 3D human cells, showing changes in replication structures under different conditions.
The EMBO Journal · Oct 7, 2025Peer-reviewedCell Biology