Identification of post-transcriptional modifications in nucleic acid sequences using purpose-designed molecular beacons
Parul Sahu, Getulio Pereira, Jian Wang, Kamil Szeliski, Nikolay V. Dokholyan, Vivian Tran, Miroslaw Korneck, John Tigges, Jennifer Clare Jones, Ionita Calin Ghiran
The paper introduces a method using molecular beacons to directly detect miRNAs and isomiRs in cells and extracellular vesicles without RNA isolation, leveraging electrophoretic mobility and computational design for m6A modifications.
bioRxiv · May 25, 2023 · PreprintMolecular Biology