John Tigges

Advancing Nano-Flow Cytometry: High-Precision Sorting and Analysis of Extracellular Vesicles

This study optimizes nanoFACS for sorting and analyzing extracellular vesicles using the CytoFLEX SRT, demonstrating its utility in isolating nanoparticles and detecting miRNA in biofluids.

Evaluating the Utility of a Nanoscale Flow Cytometer for Detection of Surface Proteins on HIV and Extracellular Vesicles

This paper evaluates the CytoFLEX nano flow cytometer's performance for detecting surface proteins on HIV and extracellular vesicles, finding it has higher scatter sensitivity and detects smaller particles and EV populations compared to a conventional flow cytometer.

Identification of post-transcriptional modifications in nucleic acid sequences using purpose-designed molecular beacons

The paper introduces a method using molecular beacons to directly detect miRNAs and isomiRs in cells and extracellular vesicles without RNA isolation, leveraging electrophoretic mobility and computational design for m6A modifications.