Improved bacterial recombineering by parallelized protein discovery
T. M. Wannier, A. Nyerges, H. Kuchwara, M. Czikkely, D. Balogh, G. T. Filsinger, N. C. Borders, C. J. Gregg, M. J. Lajoie, X. Rios, C. Pal, G. M. Church
This paper introduces a high-throughput method called SEER to discover efficient single-stranded DNA-annealing proteins (SSAPs) for bacterial recombineering, identifying variants that significantly improve genome editing efficiency in multiple bacterial species.
Proceedings of the National Academy of Sciences · May 28, 2020Peer-reviewedMicrobiology