C. Pal

Pathogen genomic surveillance as a scalable framework for precision phage therapy

This paper develops a scalable framework for precision phage therapy by combining genomic surveillance and phage typing to target carbapenem-resistant Acinetobacter baumannii.

Enzyme Fragment Complementation Driven by Nucleic Acid Hybridization

This paper introduces a biosensor for detecting nucleic acid interactions using modified protein fragment complementation with NanoBiT fragments conjugated to steramers.

Antibiotics of the future are prone to resistance in Gram-negative pathogens

This study shows new antibiotics are as likely to face resistance as existing ones in Gram-negative bacteria, highlighting the need for narrow-spectrum therapies.

Metabolic complexity increases adaptability

This study shows that metabolic complexity enhances adaptability in bacteria, as generalist E. coli strains require fewer new reactions to adapt than specialized endosymbionts.

The evolution of colistin resistance increases bacterial resistance to host antimicrobial peptides and virulence

This study shows that colistin resistance in E. coli increases resistance to host antimicrobial peptides and virulence, highlighting risks of therapeutic AMP use.

Negative trade-off between neoantigen repertoire breadth and the specificity of HLA-I molecules shapes antitumour immunity

The paper finds that HLA-I alleles with high peptide binding promiscuity are associated with worse prognosis in cancer patients treated with immune checkpoint inhibitors.

Improved bacterial recombineering by parallelized protein discovery

This paper introduces a high-throughput method called SEER to discover efficient single-stranded DNA-annealing proteins (SSAPs) for bacterial recombineering, identifying variants that significantly improve genome editing efficiency in multiple bacterial species.

Mismatch repair hierarchy of Pseudomonas putida revealed by mutagenic ssDNA recombineering of the pyrF gene

The paper investigates the mismatch repair hierarchy in Pseudomonas putida using mutagenic ssDNA recombineering of the pyrF gene, revealing a specific order of mismatch recognition.

Chemical-genetic profiling reveals cross-resistance and collateral sensitivity between antimicrobial peptides

This study uses a chemical-genetic approach to analyze resistance determinants of E. coli to 15 AMPs, revealing cross-resistance and collateral sensitivity patterns.

Antibiotic usage promotes the evolution of resistance against gepotidacin, a novel multi-targeting drug

This study shows that antibiotic use promotes resistance evolution against gepotidacin, a multi-targeting antibiotic, through mutations that also confer resistance to fluoroquinolones.

Phylogenetic barriers to horizontal transfer of antimicrobial peptide resistance genes in the human gut microbiota

This study investigates the horizontal transfer of antimicrobial peptide resistance genes in the human gut microbiota, finding that their transfer rates and functional compatibility are lower than antibiotic resistance genes.

Pathogen diversity drives the evolution of promiscuous peptide binding of human MHC-II alleles

This study shows that pathogen diversity drives the evolution of promiscuous peptide binding in human MHC-II alleles, explaining their global distribution and immune function.