TnClone: high-throughput clonal analysis usingTn5-mediated library construction and de novo assembly
Byungjin Hwang, Sunghoon Heo, Namjin Cho, Hanna Seo, Duhee Bang
TnClone reduces cloning validation costs by 20-fold using NGS and detects low-frequency mutants with 99.4% sensitivity.
ACS Synthetic Biology · Feb 6, 2019Peer-reviewedSynthetic Biology