Byungjin Hwang

The paper evaluates how biological prior knowledge aligns with perturbation responses and its impact on predictive performance in prior-informed models.

This paper introduces SCITO-Perturb-seq, a CRISPRa platform that maps surface protein regulation in CD4 T cells, finding 16% of activated genes alter surface protein expression.

SCITO-seq enables cost-effective profiling of over 10^5 cells using combinatorial indexing with dsc-seq, demonstrating feasibility in mixed cell lines and human lymphocytes.

This paper develops a genetic barcode system using a Cas9-deaminase to trace cell lineage in mammals, showing accurate reconstruction of lineage from bulk and single-cell data.

TnClone reduces cloning validation costs by 20-fold using NGS and detects low-frequency mutants with 99.4% sensitivity.