Purification of Cross-linked RNA-Protein Complexes by Phenol-Toluol Extraction
Erika C. Urdaneta, Carlos H. Vieira-Vieira, Timon Hick, Hans-Herrmann Wessels, Davide Figini, Rebecca Moschall, Jan Medenbach, Uwe Ohler, Sander Granneman, Matthias Selbach, Benedikt M. Beckmann
The paper introduces a new method, PTex, to purify RNA-protein complexes without relying on polyadenylated RNA, and provides a global RNA-bound proteome for human and bacterial cells.
Nature Communications · Mar 1, 2019Peer-reviewedMolecular Biology