Matthias Selbach

The paper introduces MEMBRYO, a method for continuous SILAC proteomics in live embryos, revealing post-transcriptional regulation during neurogenesis.

The paper integrates mRNA and protein isoform data in human RPE-1 cells to reveal widespread translation of alternative transcripts and proteolytic processing as a major source of proteome complexity.

The paper quantifies gene expression noise in mammalian cells using single-cell proteomics and finds that noise is largely extrinsic and structured across cellular states.

This study reveals that autophagy selectively clears the endoplasmic reticulum during inflammation-induced muscle atrophy, challenging the classical view of atrophy as a uniform degradation process.

The paper introduces SPIED-DIA, a phosphoproteomics method, to identify synergistic signaling responses in colon cancer cells to MEK inhibition and growth factor stimulation.

This study identifies a novel role for SORLA in regulating exosome release and neurotrophic function, linking SORL1 mutations to Alzheimer's disease pathology.

The paper establishes an ALS hiPSC model showing axonal downregulation of neuromuscular junction proteins and identifies FUS binding to their mRNAs.

This study identifies striatin-3 as a Rac1 interactor and shows its role in Schwann cell radial sorting and Hippo pathway regulation during peripheral nervous system development.

This paper introduces DIA-SiS, a method combining DIA with spike-in SILAC to improve proteome coverage and quantification, showing better performance than label-free approaches.

The paper quantifies proteomic effects of aneuploidy in natural yeast isolates, finding chromosome-wide dosage compensation in the proteome but not transcriptome.

The paper investigates how pathogenic mutations at phosphorylation sites affect protein-protein interactions, showing that such mutations disrupt phosphorylation-dependent motifs and alter cellular functions.

The paper measures translation dynamics in mouse neocortex neurogenesis, revealing a critical period of translational control that regulates protein expression and neuronal differentiation.

The paper introduces iKiP-DB, a database that predicts kinase activity from phosphoproteomic data, showing its effectiveness in analyzing both well-known and understudied kinases.

The paper investigates PGBD1's role in neuronal progenitor identity, stress sensing, and mammal-specific paraspeckles, showing its dual function in DNA binding and protein interaction.

The paper uses bulk and single-cell transcriptomics to analyze SARS-CoV-2 infection in human cell lines, identifying HSP90 as a potential therapeutic target.

The paper reveals Ebp1's role in neuronal proteostasis by analyzing ribosomal architecture and proteomic changes in the developing mouse neocortex.

This study identifies HMGXB4 as a regulator of Sleeping Beauty transposition in vertebrate germinal stem cells, linking it to chromatin dynamics and stemness maintenance during early development.

This study identifies M segment RNA splicing as a determinant of host range in influenza A virus by comparing human- and bird-adapted strains.

The paper shows Wnt11/Fzd7 signaling regulates L-type Ca2+ channels by compartmentalizing AKAP2/PKA, essential for heart development in zebrafish.

The paper introduces a new method, PTex, to purify RNA-protein complexes without relying on polyadenylated RNA, and provides a global RNA-bound proteome for human and bacterial cells.

The paper identifies and characterizes conserved small ORFs in multiple animal species, finding novel micropeptides with evidence of translation and functional potential.

RiboTaper identifies actively translated open reading frames using spectral analysis of Ribo-seq data, revealing novel ORFs and validating peptide products in HEK293 cells.