The REFLEX system enables in vivo identification of perivascular angiogenic macrophages in the heart
Tatsuyuki Sato, Takayuki Isagawa, Toshinaru Kawakami, Sosuke Hosokawa, Masamichi Ito, Daigo Sawaki, Shigeru Sato, Yu Nakagama, Kazutoshi Ono, Ariunbold Chuluun-Erdene, Thuc Toan Pham, Ryohei Tanaka, Atsumasa Kurozumi, Hiroaki Semba, Masaki Wake, , Norihiko Takeda
The paper develops REFLEX and HUNTERuni-seq to identify perivascular angiogenic macrophages (PVAMs) that deliver VEGF-A to endothelial cells, showing physical contact is critical for effective signaling.
bioRxiv · Jan 30, 2026 · PreprintCell Biology