Validated CRISPR/Cas9 guide RNAs targeting neurodevelopmental genes in the tunicate Ciona robusta
Sydney Popsuj, Tenzin Kalsang, Kwantae Kim, Erica Drummond, Pooja Manekar, Pranavvarma V. Munagapati, Manasi Oleti, Hiroki Sato, Izabella Vickery, Eduardo D. Gigante, Alberto Stolfi
The paper validates CRISPR/Cas9 guide RNAs for eight neurodevelopmental genes in Ciona robusta, showing varying mutagenesis efficacy and improved correlation with RS3 scores.
Differentiation · Jun 7, 2026Peer-reviewedDevelopmental Biology