Unraveling the Mechanism of a LOV Domain Optogenetic Sensor: A Glutamine Lever Induces Unfolding of the Jα Helix
J. N. Iuliano, J. Tolentino Collado, A. A. Gil, P. V. Ravindran, A. Lukacs, S. Shin, H. A. Woroniecka, K. Adamczyk, J. M. Aramini, U. R. Edupuganti, C. R. Hall, G. M. Greetham, I. V. Sazanovich, I. P. Clark, T. Daryaee, , P. J. Tonge
This study investigates the structural dynamics of the LOV2 domain in AsLOV2, showing that Q513 and N414 mediate J-helix unfolding linked to optogenetic function.
bioRxiv · Jan 29, 2020Biophysics