Gene-sized DNA insertion at genomic safe harbors in human cells using a site-directed transposase
James E Short, Lisa Sharek, Joshua F. Meckler, Ilko Stoytchev, Christopher T Tran, Clara Errard, Brian E. Hew, Brandon E. Johnson, David F. Waller, Shanfu Xie, Joseph J Higgins, Raymond Tabibiazar, Jesse B. Owens
This paper develops a site-directed transposase system for precise genomic insertion of gene-sized DNA without double-strand breaks, achieving on-target insertion with minimal off-target effects.
Nucleic Acids Research · Nov 5, 2025Peer-reviewedMolecular Biology