Biochemical characterization of Bacillus anthracis sortase B: Use in sortase mediated ligation and substrate recognition dependent on residues beyond the canonical pentapeptide binding motif for sortase enzymes
S. N. Jackson, J. M. Blount, K. A. Croney, D. E. Lee, J. W. Ibershof, K. M. Whitham, J. McCarty, J. M. Antos, J. Amacher
This study characterizes Bacillus anthracis SrtB, showing that residues outside the canonical pentapeptide motif are critical for activity and that engineered variants can improve ligation efficiency.
bioRxiv · May 7, 2024Biochemistry