Morphogen-driven human iPSCs differentiation in 3D in vitro models of gastrulation is precluded by physical confinement.
H. S. Alsehli, E. Roy, T. Williams, A. Kuziola, Y. Guo, J. B. Green, E. Gentleman, D. Danovi
This study shows that physical confinement reduces SOX17 expression in hiPSCs spheroids during gastrulation, but time-softening hydrogels rescue this effect.
bioRxiv · Mar 29, 2023 · PreprintCell Biology