J. M. Borrero-de Acuna

This study introduces a method to quantify bacterial membrane vesicles using FM4-64 dye and linear regression, showing its superiority over protein-based methods and identifying a key regulator in vesicle-mediated pathogenesis.

The study shows that the C. difficile strain 630Δerm has a mutation in PerR that causes oxidative stress response under anaerobic conditions, challenging its use as a model strain.