Isolation and quantification of bacterial membrane vesicles for quantitative metabolic studies using mammalian cell cultures
A. Wegner, M. Kretschmer, J. Mueller, V. Otto, A. A. Rodriguez, M. Muesken, D. Jahn, J. M. Borrero-de Acuna, M. Neumann-Schaal, P. Henke
This study introduces a method to quantify bacterial membrane vesicles using FM4-64 dye and linear regression, showing its superiority over protein-based methods and identifying a key regulator in vesicle-mediated pathogenesis.
bioRxiv · Sep 25, 2023Cell Biology