J. R. Winther

A suicidal and extensively disordered luciferase with a bright luminescence

This paper investigates the structure and mechanism of Gaussia luciferase, revealing its disordered structure and identifying key residues for light production and inactivation.

Increasing protein stability by inferring substitution effects from high-throughput experiments

The paper uses a computational model to infer substitution effects from high-throughput experiments, increasing protein stability through targeted amino acid changes.

Prolonging coagulant activity of factor Xa under hemophilic conditions by site-specific N-glycosylation of the surface-exposed autolysis loop

This study shows that N-glycosylation of FX's autolysis loop reduces FXa activation but enhances procoagulant activity in plasma assays.

Computational and experimental assessment of backbone templates for computational protein design

The paper assesses computational protein design templates using Rosetta Design and experimental validation, finding that the cart_bonded energy term improves template selection for successful designs.

Global analysis of multi-mutants to improveprotein function

This paper introduces GMMA, a method to identify beneficial amino acid substitutions in proteins using multi-mutant data, and applies it to GFP variants to find stabilizing and functional improvements.

Charge Interactions in a Highly Charge-depleted Protein

This study investigates charge interactions in a charge-depleted protein using simulations and experiments, revealing context-dependent residue interactions and their impact on protein stability.