Single-cell transcriptomics defines an improved, validated monoculture protocol for differentiation of human iPSCs to microglia
S. J. Washer, M. Perez-Alcantara, Y. Chen, J. Steer, W. S. James, A. R. Bassett, S. A. Cowley
The paper identifies redundant medium components in iPSC differentiation to microglia using single-cell RNA sequencing and provides an optimized protocol for generating functional microglia.
bioRxiv · Aug 2, 2022Immunology