Kinetic and structural characterization of the self-labeling protein tags HaloTag7, SNAP-tag and CLIP-tag
J. Wilhelm, S. Kuehn, M. Tarnawski, G. Gotthard, J. Tuennermann, T. Taenzer, J. Karpenko, N. Mertes, L. Xue, U. Uhrig, J. Reinstein, J. Hiblot, K. Johnsson
The paper compares the labeling kinetics and substrate specificities of HaloTag7, SNAP-tag, and CLIP-tag to guide their optimal use in bioimaging and biotechnology.
bioRxiv · Apr 13, 2021Biochemistry